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General Comment |
Buanne P, et al 2000 reported the cloning of PC3B, a novel member of the PC3/BTG/TOB family of
growth inhibitory genes, highly expressed in the olfactory
epithelium.
They identified in the EST database murine and human sequences similar,, but not
identical, to the members of the PC3/BTG/TOB family of cell cycle inhibitors.
A conserved domain (aa 50-68) of the PC3 protein, the prototype member of the
family, was used as a query. That domain has been shown by us to be necessary
for the antiproliferative activity of PC3. A murine EST clone and a highly
homologous human EST clone, containing the entire ORF, were chosen for
sequencing. Comparison to databases and a phylogenetic tree analysis indicated
that these EST clones are the mouse and human homologues of a gene that
represents a novel member of the PC3/BTG/TOB family. This gene, named PC3B, is
endowed with marked antiproliferative activity, being able to induce G(1)
arrest, and is highly expressed in testis, in oocyte, and in preimplantation
embryos.
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