The SRY and SOX proteins share a DNA-binding domain known as the HMG box, defined by a 79-amino acid
region. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending
of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and
interactions of other transcription factors.
NCBI Summary:
The testis-determining gene SRY (MIM 480000) encodes a transcription factor characterized by a DNA-binding motif known as the HMG (high mobility group) box. The SOX gene family consists of genes related to SRY, with a sequence identity of more than 60% to the SRY HMG box. See MIM 600898.[supplied by OMIM]
General function
Nucleic acid binding, DNA binding, Transcription factor
Comment
Cellular localization
Nuclear
Comment
Ovarian function
Comment
Kido S, et al reported the cloning and characterization of mouse mSox13 cDNA.
A novel SRY-related cDNA, mSox13, was isolated from a lambda phage library
derived from mouse embryo. The cDNA encodes a protein of 595 amino acids
containing the SRY-type high mobility group (HMG) box and a putative leucine zipper
motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that
the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named
Q box, are well conserved among known type-D SOX proteins. The expression of
mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay
indicates that the recombinant mSox13 protein is capable of binding to the AACAAT
sequence.