:
Graham KM,et al reported the expression of an intracisternal A-particle-like element in rat
ovary.
They have isolated a rat intracisternal-A particle element (IAP)-like element
(LAP-LE) from ovarian granulosa cells that appears to be identical to the rat
EST clone AA964260. The compiled cDNA sequences contain several putative
in-frame translation initiation codons with the largest capable of encoding a
365 amino acid protein with a reverse transcriptase domain in the N-terminus
as well as a bipartite nuclear localization signal sequence in the middle.
Northern blotting shows a major similar to7 Kb transcript and a minor similar
to5 Kb transcript that are abundantly expressed in the ovary. In situ
hybridization histochemistry using ovaries from gonadotropin-treated immature
rats and regularly cycling adult rats show that this transcript is
predominantly localized to granulosa cells of all healthy follicles, including
primary follicles, and to newly-formed and healthy corpora lutea. This
cell-specific expression pattern of the IAP-LE gene is distinct from those of
the several known retroviral elements, suggesting the potentially novel
functional importance of the IAP-LE gene.
General function
Comment
Cellular localization
Nuclear
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Ovarian function
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Expression regulated by
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Ovarian localization
Granulosa, Luteal cells
Comment
Follicle stages
Primary, Secondary, Antral, Preovulatory, Corpus luteum