Chromosomal protein HMG14 and its close analog HMG17 bind to the inner side of the
nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be
involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous
distribution, relative abundance, and high evolutionary conservation of the DNA-binding domain of the HMG-14 family
of proteins, suggest that they may be involved in an important cellular function. The human HMG14 multigene family is
1 of the largest retropseudogene families known.
NCBI Summary:
Member of the HMG 14/17 family of proteins; affects rate of elongation by RNA polymerase II on chromatin templates
General function
Cell death/survival, DNA Replication, Nucleic acid binding, DNA binding, Transcription factor
Comment
Cellular localization
Nuclear
Comment
Ovarian function
Oogenesis
Comment
Mohamed OA, et al 2001 reported that
High-mobility group proteins 14 and 17 maintain the timing of
early embryonic development in the mouse.
The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal
proteins that bind to nucleosomes and enhance transcription. The authors reported that
both mRNA species and both proteins are present throughout oogenesis and
preimplantation development of the mouse.. The results identify HMG-14 and HMG-17 as constitutive components
of mouse oocyte and embryonic chromatin and establish a link between the
structure of embryonic chromatin and the normal progression of embryonic
development.
Expression regulated by
Comment
Ovarian localization
Oocyte
Comment
Follicle stages
Antral, Preovulatory
Comment
Liu HC, et al 2001 reported tha application of complementary DNA microarray (DNA chip) technology in the study of gene expression profiles during
folliculogenesis.
They used oligonucleotide microarray (DNA chip)-based hybridization
analysis to gain a comprehensive view of gene expression and regulation
involved in folliculogenesis.
Preantral follicles isolated from day 14 B6D2F-1 mice
were stimulated in vitro to form Graafian follicles. Total RNA extracted from
the mouse preantral and Graafian follicles were reverse transcribed, labeled
with digoxigenin-11-dUTP, and then hybridized with Clontech Atlas mouse cDNA
expression arrays for comparison. Of 588 known studied genes, 39 and 61 were detected in preantral follicles and in Graafian follicles, respectively, and 17 were highly
expressed consistently in both preantral and Graafian follicles. Performing
clustering analysis, 46 were upregulated as the follicles advanced to mature stages.
The HIGH MOBILITY GROUP PROTEIN 14 is up-regulated in the Graafian follicles.