Basigin is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. It was cloned as a carrier of an
oncodevelopmental carbohydrate marker expressed in teratocarcinoma stem cells. It is
expressed broadly in both embryos and adults. As members of the immunoglobulin superfamily play
fundamental roles in intercellular recognition involved in various immunologic
phenomena, differentiation, and development, basigin is thought also to play a role in
intercellular recognition.
NCBI Summary:
The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]
General function
Cell adhesion molecule
Comment
Cellular localization
Extracellular Matrix, Secreted
Comment
Ovarian function
Luteinization, Oogenesis
Comment
Expression of Basigin, an Inducer of Matrix Metalloproteinases, in the Rat Ovary McDonnel Smedts A,et al .
The extensive tissue remodeling that occurs during follicular development, ovulatory rupture, and the formation and regression of the corpus luteum (CL) requires local degradation of the extracellular environment by matrix metalloproteinases (MMPs). This report characterizes the expression pattern of basigin (Bsg), a putative regulator of MMP induction, in the rat ovary. An induced superovulation model (eCG/hCG) was employed in immature rats to evaluate Bsg expression profiles in ovaries collected during the follicular phase, the pre-ovulatory period, and the luteal lifespan. Levels of Bsg mRNA were unchanged through follicular growth (0 - 48 h post-eCG) and increased during post-ovulatory luteinization (24 and 48 h post-hCG; P < 0.01). Bsg expression persisted into pseudopregnancy (4 - 8 d post-hCG) and after functional luteal regression (12 d post-hCG). The profile of Bsg expression during regression of the CL was examined utilizing a model of induced luteolysis. Both functional and structural regression were associated with a decline in Bsg expression levels. Bsg mRNA and protein localized to the theca of preovulatory follicles (12 h post-hCG) and formative and functional CL (24 h - 8 d post-hCG). Bsg expression profiles in the induced-ovulation/CL regression models were similar to observations made in naturally cycling mature rats. In the cycling ovary, Bsg signaling localized to newly forming CL, the theca of preovulatory follicles, and appeared to be lower in CL from previous estrous cycles. A putative regulatory mechanism of Bsg expression was identified utilizing an in vitro model; treatment of cultured granulosa cells with hCG significantly augmented Bsg mRNA expression levels. The processes of ovulation and luteogenesis may be facilitated by Bsg expression and its induction/regulation of the MMPs.
SARS-CoV-2 host receptors ACE2 and CD147 (BSG) are present on human oocytes and blastocysts. Essahib W et al. (2020) To visualize SARS-CoV-2 host receptors ACE2 and CD147 on human oocytes and blastocysts. Immunohistochemistry and confocal microscopy on human primary oocytes and pre (5 days post fertilization (dpf5) and (dpf6))- and peri (dpf7)-implantation blastocysts donated to research. SARS-CoV-2 host receptors ACE2 and CD147 are present on the membrane of trophectoderm, epiblast and hypoblast cells in human blastocysts. CD147 is also present on the oolemma. Theoretically, the earliest stages of embryonic development may be vulnerable for SARS-CoV-2 infection.//////////////////
Can follicular Emmprin and BMP 4 levels predict ICSI outcome? Takmaz O et al. (2019) To evaluate the relationship of clinical pregnancy rates with bone morphogenetic proteins 2-4-7 (BMP 2, 4, 7), growth differentiation factor 9 (GDF 9), and Emmprin levels in follicular fluid of intracytoplasmic sperm injection patients. Follicular fluid of 77 patients who underwent ICSI procedure was collected during the oocyte retrieval procedure. And follicular fluid levels of BMP 2, BMP 4, BMP 7, GDF 9, and Emmprin (Basigin) were measured and compared for clinical pregnancy rates. Follicular levels of BMP 4 was significantly higher whereas Emmprin levels were lower in patients who had achieved clinically diagnosed pregnancy compared with those who did not achieve clinical pregnancy after ICSI procedure (P = 0.007 and P = 0.035, respectively). BMP 2, BMP 7, and GDF 9 levels were comparable for both groups. Clinical pregnancy rates after ICSI may be associated with follicular fluid levels of Emmprin and BMP 4. Follicular levels of Emmprin and BMP 4 can be used as a marker (as markers for predicting ICSI outcomes) for a better ICSI outcome.//////////////////
Ding NZ, et al reported the quantification of basigin mRNA in mouse oocytes and
preimplantation embryos by competitive RT-PCR.
The aim of this study was to
use a quantitative competitive polymerase chain reaction to assess
quantitatively the levels of basigin mRNA in mouse oocyte and preimplantation
embryos. Basigin mRNA was detected in the oocyte and all the stages of
preimplantation embryos. The levels of basigin mRNA were 0.0606 +/- 0.0282 in
the oocyte, 0.0102 +/- 0.0036 in the zygote, 0.0007 +/- 0.0003 in the 2-cell
embryo, 0.0031 +/- 0.0017 in the 4-cell embryo, 0.0084 +/- 0.0024 in the
8-cell embryo, 0.0537 +/- 0.0121 in the morula and 0.0392 +/- 0.0161 attomoles
in the blastocyst, respectively. The levels of basigin mRNA in the oocyte,
morula and blastocyst were significantly higher than those in the zygote and
embryos at the 2-cell, 4-cell and 8-cell stages. The high level of basigin
expression in the blastocyst may play a role during embryo implantation.
Expression of an extracellular matrix metalloproteinase inducer (basigin) in the human ovary and ovarian endometriosis. Smedts AM et al. OBJECTIVE: To characterize the expression of basigin in ovarian endometriotic tissue, normal ovary, and endometrium during the menstrual cycle. DESIGN: Controlled, retrospective study for evaluation of basigin expression in archived specimens. SETTING: Academic research environment: University of Kentucky College of Medicine. PATIENT(S): Archived surgical specimens selected by retrospective chart review of age-matched (39 +/- 2.5 years) patients with and without ovarian endometriosis. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Expression localization of basigin mRNA and protein. RESULT(S): Basigin (mRNA and protein) was localized to granulosa cells (follicles of all stages), the ovarian surface epithelium, and granulosa and theca lutein of corpora lutea. Differential patterns of expression were apparent among samples of eutopic endometrium collected during the proliferative and secretory stages. In endometriotic lesions on the ovary, basigin was detected in the glandular epithelia and stroma of proliferative and secretory phase samples, but patterns of expression were unsynchronized with those of the eutopic endometrium from the same patient. CONCLUSION(S): The current data demonstrate unique expression patterns of basigin in the human ovary and endometriotic tissue, thereby supporting a possible role in normal ovarian function and in the dysregulation of proteolytic matrix metalloproteinases in endometriosis.
Follicle stages
Antral, Preovulatory, Corpus luteum
Comment
Chang H, et al 2004 reported Basigin expression and regulation in mouse ovary during the sexual maturation and development of corpus luteum.
Basigin is a highly glycosylated transmembrane protein belonging to the immunoglobulin superfamily. Basigin-deficient male mice are azoospermic. The majority of basigin null embryos die around the time of implantation. However, basigin expression and regulation in mouse ovary is still unknown. The aim of this study was to investigate basigin expression in mouse ovary during sexual maturation, gonadotropin treatment, and luteal development by in situ hybridization and immunohistochemistry. Both basigin mRNA and immunostaining were not detected in the granulosa cells of preantral follicles until day 20 after birth. On day 30 after birth, basigin immunostaining dropped to a basal level, while basigin mRNA was still at a high level. Basigin expression was strongly induced by equine chorionic gonadotropin (eCG) treatment at 4 and 8 hr post-eCG injection. Both basigin immunostaining and mRNA signals were strongly observed in the corpus luteum on days 2 and 3 post-hCG injection. However, no basigin expression was detected from days 6 to 15 post-hCG injection. In conclusion, our data suggest that basigin may play a role during the mouse follicle development and corpus luteum formation.
Phenotypes
Mutations
1 mutations
Species: mouse
Mutation name: None
type: null mutation fertility: infertile - ovarian defect Comment:Kuno N, et al 1998 reported female sterility in mice lacking the basigin gene, which encodes a
transmembrane glycoprotein belonging to the immunoglobulin
superfamily.
Basigin (Bsg) is a transmembrane glycoprotein belonging to the immunoglobulin
superfamily. Bsg knock-out mice exhibit infertility of both sexes. Based on limited
results, defective implantation has been considered to be the cause of the female
infertility. The authors found that disruption of the Bsg gene produces the
failure of female reproductive processes including not only implantation but also
fertilization. Bsg mRNA expression in cumulus cells and basolateral localization
of the Bsg protein in the endometrial epithelium further support the importance of
Bsg in these processes.