The protein encoded by the tsg101 cDNA contains a coiled-coil domain that interacts with stathmin (OMIM 151442), a
cytosolic phosphoprotein implicated in tumorigenesis. Li and Cohen (1996) observed that overexpression of tsg101
antisense transcripts in NIH 3T3 cells resulted in cell transformation and increased stathmin-specific mRNA.By database searching and comparison of the TSG101 proteins of yeast and other organisms, Koonin and Abagyan
(1997) concluded that TSG101 may belong to a group of apparently inactive homologs of ubiquitin-conjugating
enzymes.
NCBI Summary:
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
General function
Tumor suppressor
Comment
TSG101 is mutated at high frequency in human breast cancer and defects in
TSG101 occur during breast cancer tumorigenesis and/or progression.
Cellular localization
Nuclear
Comment
Ovarian function
Comment
Expression regulated by
Comment
Ovarian localization
Comment
Follicle stages
Antral, Preovulatory
Comment
Liu HC, et al 2001 reported tha application of complementary DNA microarray (DNA chip) technology in the study of gene expression profiles during
folliculogenesis.
They used oligonucleotide microarray (DNA chip)-based hybridization
analysis to gain a comprehensive view of gene expression and regulation
involved in folliculogenesis.
Preantral follicles isolated from day 14 B6D2F-1 mice
were stimulated in vitro to form Graafian follicles. Total RNA extracted from
the mouse preantral and Graafian follicles were reverse transcribed, labeled
with digoxigenin-11-dUTP, and then hybridized with Clontech Atlas mouse cDNA
expression arrays for comparison. Of 588 known studied genes, 39 and 61 were detected in preantral follicles and in Graafian follicles, respectively, and 17 were highly
expressed consistently in both preantral and Graafian follicles, including Tumor Susceptibility Gene 101.