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MYOD FAMILY INHIBITOR; MDFI OKDB#: 1329
 Symbols: MYOD FAMILY INHIBITOR; MDFI Species: human
 Synonyms:  Locus: 6p21 in Homo sapiens


For retrieval of Nucleotide and Amino Acid sequences please go to: OMIM Entrez Gene
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R-L INTERACTIONS   MGI

DNA Microarrays
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link to BioGPS
General Comment Mdfi inhibits the transactivation activity of MyoD family members and represses myogenesis. Immunofluorescence microscopy revealed that Mdfi associates with MyoD family members in the cytoplasm and retains them by masking their nuclear localization signals.

NCBI Summary: This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interfers with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.
General function Intracellular protein transport
Comment
Cellular localization Cytoplasmic
Comment
Ovarian function Follicle development
Comment Tetsuya Mizutani et al 2001 reported the cloning and Characterization of Gonadotropin-Inducible Ovarian Transcription Factors (GIOT1 and -2) That Are Novel Members of the (Cys)2-(His)2-Type Zinc Finger Protein Family. Gonadotropins are essential for ovarian follicular development and differentiation. To identify genes that are rapidly induced by gonadotropin in the immature rat ovary, ovarian genes were screened by a subtraction cloning procedure. cDNA clones encoding novel members of the (Cys)2-(His)2-type zinc finger protein family GIOT1 and -2 (gonadotropin-inducible transcription factor 1 and 2), were identified. Two isoforms of GIOT2 (GIOT2 and 2), which are probably produced by alternative splicing, also exist. Nucleotide sequence analysis revealed that GIOT1, but not GIOT2, contains the krppel-associated box-A domain at the NH2 terminus. RNA analyses revealed that these mRNAs were rapidly and temporarily induced by gonadotropins in the rat testis as well as in the ovary. In situ hybridization study revealed that expression of GIOT1 was induced in theca interna cells in the ovary and Leydig cells in the testis. Interestingly, the gene expression of GIOT1 is restricted to the pituitary, adrenal, testis, and ovary, while GIOT2 gene is expressed ubiquitously. A functional analysis of GIOT1 and -2 by a GAL4-based mammalian one-hybrid system revealed that GIOT1, but not GIOT2, is a transcriptional repressor and that the krppel-associated box-A domain of GIOT1 is responsible for the transcriptional repressor activity. A GAL4-based yeast two-hybrid system was also used to identify proteins that interact with the rat GIOT1. The authors cloned genes encoding rat homologs of human I-mfa domain containing protein and transcriptional intermediary factor 1 , both of which are transcription-regulatory proteins. Interaction of these proteins with GIOT1 was directly demonstrated by GST pull-down assay. The data strongly suggest that GIOT1 may function as a novel transcriptional repressor by working with rat homologs of human I-mfa domain containing protein and transcriptional intermediary factor 1 proteins and may play a significant role at the transcription level in the folliculogenesis.
Expression regulated by
Comment
Ovarian localization
Comment
Follicle stages
Comment
Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Links
OMIM (Online Mendelian Inheritance in Man: an excellent source of general gene description and genetic information.)
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created: Oct. 3, 2001, 10:40 a.m. by: hsueh   email:
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last update: Oct. 3, 2001, 10:40 a.m. by: system    email:



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