Torigoe et al. (1997) purified human IL18R, symbolized IL18R1, by selecting a Hodgkin disease cell line, L428, that
was the best binder in IL18 binding assays. //////involved in pyroptosis?
NCBI Summary:
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
General function
Receptor
Comment
Cellular localization
Plasma membrane
Comment
Ovarian function
Ovulation, Oogenesis
Comment
Tsuji Y, et al reported the expression of interleukin-18 and its receptor in mouse ovary.
Interleukin-18 (IL-18) strongly induces interferon-gamma production
and is produced not only by types of immune cells but also by types of
non-immune cells. Ovulation is thought to be an inflammation-like reaction in
which many pro-inflammatory cytokines are involved.
4-week-old immature female mice were examined for IL-18 and IL-18 receptor
(IL-18R) expression on their ovaries under stimulation with pregnant mare's
serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) by
immunohistochemical staining, Western blotting and reverse
transcript-polymerase chain reaction indicated the
expression of both proteins and mRNA of IL-18 and IL-18R were very low in
immature ovaries before stimulation, but after PMSG injection both IL-18 and
IL-18R increased dramatically in theca cells and reached a maximum level at the
peri-ovulatory phase then slightly lowered, but still kept a high level during the
luteal phase in the corpus luteum. The treatment of IL-18R monoclonal antibody
to the mice during ovarian stimulation reduced the number of ovulated ova and
inhibited the expansion of cumulus cells surrounding the ovum.
Expression regulated by
FSH
Comment
Ovarian localization
Granulosa, Theca, Luteal cells
Comment
Expression of mRNA and protein of IL-18 and its receptor in human follicular granulosa cells. Salmassi A et al. (2016) There is no information available about the IL-18 receptor in ovarian follicles, so the present study attempts to demonstrate the expression of IL-18 and its receptor in human granulosa cells (GCs). To evaluate the concentration of IL-18 in serum and follicular fluid (FF), we collected serum and FF from 102 women undergoing oocyte retrieval. Also, to detect expression of IL-18 and its receptor by luteinized GCs, these cells were pooled six times from a total of twenty individual patients with 5-16 follicles each. The IL-18 concentration was determined by ELISA and the expression of IL-18 and its receptor by immunocytochemistry and reverse transcription polymerase chain reaction. Our results showed that the median IL-18 concentration in serum, 159.27 pg/ml (IQR 121.41-210.1), was significantly higher than in FF, 142.1 pg/ml (IQR 95.7-176.5), p < 0.001. Moreover, we found that IL-18 and its receptor are expressed by GCs. The presence of IL-18 in FF and the expression of IL-18 and its receptor by GCs suggest an important role for this cytokine in ovarian function.//////////////////