Nuclear factor of activated T cells (NFAT) is a transcription factor required for T-cell expression
of interleukin 2 (IL2; OMIM 147680). NFAT binds to a sequence known as the antigen receptor response element 2 (ARRE2) in the
IL2 enhancer. Purified NFAT contains 45- and 90-kD subunits.
NCBI Summary:
Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.
General function
Nucleic acid binding, DNA binding, Transcription factor
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Cellular localization
Nuclear, nucleolus
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Ovarian function
Oogenesis, Oocyte maturation
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Genes whose expression is detected by cDNA array hybridization: cell surface antigens, cell adhesion, receptors. Also, relative transcript level reproducibly decreases during IVM Rozenn Dalbis-Tran and Pascal Mermilloda
Expression regulated by
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Ovarian localization
Oocyte
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Lopez-Fernandez LA, et al reported that Ilf2 is regulated during meiosis and associated to transcriptionally
active chromatin.
Analysis of gene expression during testis development demonstrated accumulation of
Ilf2 mRNA in pachytene spermatocytes. In these cells, the protein was localized in the
nucleus, but it was absent from chromatin of the XY pachytene bivalent, in which
there is no transcriptional activity. Nucleolar signal is inmmunolocalized in
spermatogonia, Sertoli cells and oocytes. By in situ hybridisation, Ilf2 expression is
detected in proliferative cells of adult ovary and a defined pattern is also exhibited in
different tissues of embryos. The presence of ILF2 in active chromatin is corroborated
in NIH3T3 cultured cells after transfection with Ilf2-EGFP constructs.