Deletions at 11q23 have been found in approximately half of breast, lung, ovarian, and cervical carcinomas as well as in
melanomas, suggesting that the region harbors at least 1 tumor susceptibility gene. There are 2 distinct regions of loss of
heterozygosity (LOH) at 11q23. ZNF202 is a gene located within
LOH region 2. Alternative splicing gives rise to alpha and beta ZNF202 protein isoforms, with predicted lengths of 424 and
648 amino acids, respectively. ZNF202-beta contains 8 zinc fingers in the C-terminal half. Northern blot analysis revealed
that ZNF202 is expressed as a 4- to 4.5-kb mRNA almost exclusively in testis.
General function
DNA binding, Transcription factor
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Cellular localization
Nuclear
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Ovarian function
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Expression regulated by
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Ovarian localization
Granulosa
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Xing WR, et al 2002 reported cross talk of two Krupple transcription factors regulates
expression of the ovine FSH receptor gene
The follicle-stimulating hormone receptor (FSHR) in gonadal cells is required
for normal folliculogenesis and spermatogenesis. To understand its regulation,
we identified a CACC-box from -46 to -67 of the ovine FSHR promoter. Antibody
supershift with a 22-bp DNA probe and nuclear extract from a Sertoli cell line
demonstrated that a testis-specific zinc finger protein, ZNF202, might be one
of the binding proteins. Western blots using ZNF202 antibody and Southwestern
blot analyses with the DNA probe detected the same 60 kDa protein in both
Sertoli and ovarian granulosa cell lines. Gel shift assays also revealed that
the DNA-protein complex from Sertoli cells overexpressing the human
Ras-responsive element binding protein-1 (RREB-1) migrated the same way as the
complex containing endogenous CACC-box binding protein. Transfection studies
indicated that ZNF202 repressed ovine FSHR promoter activity whereas RREB-I
was likely to function as an activator. These data suggest that selective
expression and cross talk of functionally distinctive Krupple transcription
factors could regulate tissue- and stage-specific expression of FSHR gene.