| CDC28 PROTEIN KINASE 1B; CKS1B | OKDB#: 1791 |
| Symbols: | CDC28 PROTEIN KINASE 1B; CKS1B | Species: | human | ||
| Synonyms: | CDC2-ASSOCIATED PROTEIN CKS1, CKS1| | Locus: | 8q21 in Homo sapiens |
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| General Comment |
The Cks1 protein is a component of the Cdc28 protein kinase in the budding yeast Saccharomyces cerevisiae. There are 2 human homologs of the Cks1 gene of yeast. Designated CKS1 and CKS2, both encode proteins of 79 amino acids that share considerable homology at the amino acid level with the products of the corresponding gene in S. cerevisiae and another gene in the fission yeast Schizosaccharomyces pombe. Both human homologs were capable of rescuing a null mutation of the S. cerevisiae Cks1 gene when expressed from the S. cerevisiae GAL1 promoter. Linked to Sepharose beads, the CKS1 and CKS2 proteins could bind the CDC28/CDC2 protein kinase from both S. cerevisiae and human cells
NCBI Summary: CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. |
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| General function | Cell death/survival, Cell cycle regulation | ||||
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| Cellular localization | Nuclear | ||||
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| Ovarian function | Oogenesis, Oocyte maturation | ||||
| Comment | Gene whose expression is detected by cDNA array hybridization: oncogenes, tumor suppressors, cell cycle regulators Rozenn Dalbi?Tran and Pascal Mermilloda The influence of follicle size, FSH-enriched maturation medium, and early cleavage on bovine oocyte maternal mRNA levels. Mourot M et al. Transcription is arrested in the bovine oocyte within the first few hours of in vitro maturation, thus the stored maternal mRNAs accumulated in the oocyte are essential to sustain development until the Maternal-Zygotic Transition. In vivo matured oocytes have superior blastocyst formation rates than in vitro matured oocytes, suggesting that the mRNA content of these oocytes is of higher quality. To determine which transcripts may be associated with developmental competence, a Suppressive Subtractive Hybridization was performed between oocytes collected by ovariectomy at 6 hr post-LH surge and oocytes from slaughterhouse collected after 6 hr of maturation, resulting in a library enriched in these functionally important mRNAs. The clones were spotted onto a cDNA microarray and transcripts potentially associated with developmental competence were hybridized onto these slides. Hybridizations were performed with transcripts up-regulated in oocytes cultured for 6 hr in the presence or absence of rFSH in vitro, and secondly with transcripts up regulated in early-cleaving embryos versus those at the one-cell stage at 36 hr postfertilization. From these hybridizations, 13 candidates were selected. Their functional association with embryonic competence was validated by measuring their relative transcript levels by quantitative real-time PCR in eight different conditions: oocytes cultured with or without rFSH, early-versus late-cleaving embryos, and oocytes from different follicle sizes (1-3, 3-5, 5-8, and >8 mm of diameter). The gene candidates CCNB2, PTTG1, H2A, CKS1, PSMB2, SKIIP, CDC5L, RGS16, and PRDX1 showed a significant quantitative association with competence compared to BMP15, GDF9, CCNB1, and STK6. Mol. Reprod. Dev. (c) 2006 Wiley-Liss, Inc. | ||||
| Expression regulated by | |||||
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| Ovarian localization | Oocyte, Granulosa | ||||
| Comment | Spruck CH, et al 2003 reported that CKS1 is expressed in granulosa cells based on in situ hybridization analyses. | ||||
| Follicle stages | Antral | ||||
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| Phenotypes | |||||
| Mutations | 0 mutations | ||||
| Genomic Region | show genomic region | ||||
| Phenotypes and GWAS | show phenotypes and GWAS | ||||
| Links |
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| created: | May 2, 2003, 11:34 a.m. | by: |
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| last update: | Oct. 30, 2006, 4:39 p.m. | by: | amazinmazin email: |
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