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Comment |
Sasson R, et al 2003 reported novel genes modulated by FSH in normal and immortalized FSH-responsive
cells and new insights into the mechanism of FSH action.
Follicle-stimulating hormone (FSH) controls the development of follicle-enclosed oocytes in the
mammalian ovary by interacting with specific receptors located exclusively on granulosa cells. Its
biological activity involves stimulation of intercellular communication, intracellular signaling, and
up-regulation of steroidogenesis; the entire spectrum of genes regulated by FSH is not yet fully
characterized. The authors have established monoclonal rat FSH-responsive granulosa cell lines that
express FSH receptors at 20-fold higher rates than with primary cells, and thus increased the
probability of yielding a distinct spectrum of genes modulated by FSH. Using Affymetrix DNA
microarrays, they discovered 11 genes not reported earlier to be up-regulated by FSH and 9 genes
not reported earlier to be down-regulated by FSH. Modulation of signal transduction associated
with G-protein signaling, phosphorylation of proteins, and intracellular-extracellular ion balance
was suggested by up-regulation of decay accelerating factor GPI-form precursor (DAF), membrane
interacting protein RGS16, protein tyrosine phosphatase (PTPase), oxidative stress-inducible protein tyrosine phosphatase (OSIPTPase), and down-regulation of rat prostatic acid phosphatase
(rPAP), Na+, K+-ATPase, and protein phosphatase 1beta. Elevation in granzyme-like proteins 1
and 3, and natural killer (NK) cell protease 1 (NKP-1) along with reduction in carboxypeptidase E
indicates possible FSH-mediated preparation of the cells for apoptosis.
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