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Splicing Factor, Proline- And Glutamine-rich OKDB#: 2371
 Symbols: SFPQ Species: human
 Synonyms: POLYPYRIMIDINE TRACT-BINDING PROTEIN-ASSOCIATED SPLICING FACTOR, PSF|PTB-ASSOCIATED SPLICING FACTOR|SFPQ/TFE3 FUSION GENE, INCLUDED|  Locus: 1p34 in Homo sapiens


For retrieval of Nucleotide and Amino Acid sequences please go to: OMIM Entrez Gene
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R-L INTERACTIONS   MGI

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General Comment
General function DNA binding, Transcription factor
Comment
Cellular localization Nuclear
Comment
Ovarian function Steroid metabolism
Comment
Expression regulated by Growth Factors/ cytokines
Comment
Ovarian localization Granulosa
Comment Urban RJ, et al reported that Protein Kinase C Iota Enhances the Transcriptional Activity of the Porcine P450 Side Chain Cleavage Insulin-like Response Element. IGF-I enhances steroidogenesis in granulosa cells by stimulating the expression of the rate-limiting steroidogenic enzyme, cytochrome P450 side-chain cleavage (P450scc). This effect is mediated through an IGF response element (IGFRE) that binds polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF) and Sp1. Sp1 is essential for activation of the IGFRE while PSF functions as a repressor. Urban RJ, et al investigated mechanisms of modulation of the IGFRE by the atypical protein kinase C, PKC iota, in a porcine stable granulosa cell line, JC-410. PKC iota was found in nuclear extracts and levels were increased by IGF-I after 24 and 48 h of treatment. Immunoprecipitation experiments demonstrated that PSF and PKC iota associated with each other in nuclear extracts from JC-410 cells. Transient transfection with expression plasmids of kinase-active and kinase-deficient PKC iota isoforms enhanced transcriptional activity of the IGFRE regardless of kinase catalytic activity. Depletion of PKC iota protein by siRNA suppressed basal IGFRE activity, but did not prevent IGF-I stimulation of the IGFRE. We conclude that PKC iota enhances transcriptional activity of the porcine P450scc IGFRE independent of kinase activity by a mechanism involving protein-protein interaction with PSF.
Follicle stages
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Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Links
OMIM (Online Mendelian Inheritance in Man: an excellent source of general gene description and genetic information.)
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created: Feb. 4, 2004, 7:35 a.m. by: hsueh   email:
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last update: Feb. 4, 2004, 7:35 a.m. by: system    email:



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