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General Comment |
Molecular cloning and characterization of ZFF29: a protein containing a unique Cys 2His 2 zinc finger motif. Asano H, et al 2004 have cloned a gene, ZFF29, from human fetal liver erythroid cells. Two types of mature mRNA were identified and designated as ZFF29a and ZFF29b. In human genome the ZFF29 gene is on chromosome 9q, and the two forms are splicing variants. There is a unique transcription start site, which predicts major mRNAs composed of 2485 bases for ZFF29a and 1801 bases for ZFF29b. The anticipated mRNAs were demonstrated in K562 cells but not in any adult human tissues examined by Northern blotting. RT-PCR revealed that the ZFF29 message is present in the adult bone marrow and the ovary at a higher level than any other mouse tissues examined. These findings suggest that ZFF29 is expressed in embryonic/fetal erythroid tissues. The deduced polypeptide chains of ZFF29a and ZFF29b are composed of 306 and 350 amino acids, respectively. A unique zinc finger motif composed of two contiguous Cys 2His 2 type fingers is common in both forms of ZFF29. ZFF29 a and b are nuclear proteins and ZFF29b, but not ZFF29a, acts as an activator of erythroid gene promoters.
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