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Ovarian Kaleidoscope Database (OKdb)

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HPMR

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Histidine Triad Nucleotide-binding Protein OKDB#: 2798
 Symbols: HINT Species: human
 Synonyms: PROTEIN KINASE C INHIBITOR 1, PRKCNH1|PROTEIN KINASE C-INTERACTING PROTEIN 1, PKCI1  Locus: 5q31.2 in Homo sapiens


For retrieval of Nucleotide and Amino Acid sequences please go to: OMIM   UCSC Genome Browser   GEO Profiles new!   Amazonia (transcriptome data) new!
R-L INTERACTIONS   MGI

DNA Microarrays
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link to BioGPS
General Comment Dalbies-Tran R et al (2003) Use of heterologous complementary DNA array screening to analyze bovine oocyte transcriptome and its evolution during in vitro maturation. Physiologie de la Reproduction et des Comportements, UMR 6073 Institut National de la Recherche Agronomique/Centre National de la Recherche Scientifique/Universite Francois Rabelais de Tours, F-37380 Nouzilly, France. We have analyzed gene expression in bovine oocytes before and after in vitro maturation (IVM) using heterologous hybridization onto cDNA array. Total RNA was purified from pools of over 200 oocytes either immediately after aspiration from follicles at the surface of slaughterhouse cow ovaries or following in vitro maturation. Radiolabeled cDNA probes were generated by reverse-transcription followed by linear PCR amplification and were hybridized to Atlas human cDNA arrays. To our knowledge, this is the first report of gene expression profiling by this technology in the mammalian female germ cell. Our results demonstrate that cDNA array screening is a suitable method for analyzing the transcription pattern in oocytes. About 300 identified genes were reproducibly shown to be expressed in the bovine oocyte, the largest profile available so far in this model. The relative abundance of most messenger RNAs appeared stable during IVM. However, 70 transcripts underwent a significant differential regulation (by a factor of at least two). Their potential role in the context of oocyte maturation is discussed. Together they constitute a molecular signature of the degree of oocyte cytoplasmic maturation achieved in vitro.

General function
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Cellular localization
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Ovarian function
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Expression regulated by
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Ovarian localization Oocyte
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Follicle stages
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Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Links
OMIM (Online Mendelian Inheritance in Man: an excellent source of general gene description and genetic information.)
OMIM \ Animal Model
KEGG Pathways
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created: March 30, 2005, 10:51 p.m. by: efeifan   email:
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last update: March 31, 2005, 4:57 p.m. by: system    email:



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