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Comment |
Yoon SJ, et al reported the identification of differential gene expression in germinal vesicle vs. metaphase II mouse oocytes by using annealing control primers.
By using a new innovative technology, annealing control primer-polymerase chain reaction (ACP-PCR), we identified genes that are differentially expressed in immature GV vs. in mature MII mouse oocytes. The results of the present study will be valuable in understanding the components of oocyte maturation and provide a basis for studies of human oocyte maturation.
Among the DEGs, we recognized three genes (Pscd2, PKD2, and CSN3) that have been reported to function in the protein kinase D (PKD containing pleckstrin homology domain)COP9 signalosome (CSN) signaling pathway (9 and 10). The differential expression of these three genes in GV and MII oocytes was confirmed by quantitative real-time PCR analysis (Fig. 1C).
PKD2, a novel phorbol ester- and growth factor-stimulated protein kinase, is widely expressed in human and murine tissues and is most highly related to human PKCμ (69% identity). It contains a pleckstrin homology domain located between the duplex zinc-fingerlike motif, and a catalytic domain exhibiting all the characteristic sequence motifs of serine protein kinases
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