Stanford Home
Ovarian Kaleidoscope Database (OKdb)

Home

History

Transgenic Mouse Models

INFORGRAPHICS

Search
Submit
Update
Chroms
Browse
Admin

Hsueh lab

HPMR

Visits
since 01/2001:
176557

Solute Carrier Family 6 (neurotransmitter Transporter, Serotonin), Member 4 OKDB#: 2839
 Symbols: SLC6A4 Species: human
 Synonyms: SEROTONIN TRANSPORTER, SERT|5-@HYDROXYTRYPTAMINE TRANSPORTER, HTT  Locus: 17q11.1-q12 in Homo sapiens


For retrieval of Nucleotide and Amino Acid sequences please go to: OMIM   UCSC Genome Browser   GEO Profiles new!   Amazonia (transcriptome data) new!
R-L INTERACTIONS   MGI

DNA Microarrays
SHOW DATA ...
link to BioGPS
General Comment
General function Channel/transport protein
Comment
Cellular localization Plasma membrane
Comment
Ovarian function
Comment
Expression regulated by
Comment
Ovarian localization Oocyte, Cumulus
Comment Serotonin and Its Antidepressant-Sensitive Transport in Mouse Cumulus-Oocyte Complexes and Early Embryos Amireault P et al . Serotonin (5-hydroxytryptamine, 5-HT), a neurohormone found in various non-neural tissues, including the gonads of many invertebrates, regulates spawning and oocyte meiotic maturation. The possibility that a local serotonergic network might also exist in the female gonads of vertebrate species, including mammals, remains poorly documented. To clarify this possibility, we investigated mouse cumulus cells, oocytes and embryos for 3 key serotonergic components, namely 5-HT itself, the rate-limiting enzyme for its production, tryptophan hydroxylase 1 (TPH1), and the 5-HT-specific transporter (SERT) required for modulating its cellular effects. Using a combination of reverse transcription-polymerase chain reaction analysis and immunofluorescence confocal microscopy, we showed that mouse cumulus cells, oocytes and embryos contain 5-HT and SERT, while only cumulus cells possess the 5-HT-producing enzyme TPH1 and may thus be the local source of 5-HT observed in other cells. With a semi-quantitative assay in single cells, we demonstrated that 5-HT can actively be taken up by isolated oocytes when it is supplied exogenously in vitro. This 5-HT transport in isolated oocytes is driven by a classical SERT, expressed up to the blastocyst stage, that is sensitive to the antidepressants fluoxetine and fluvoxamine, that belong to the selective serotonin reuptake inhibitor family. Altogether, our results show that 5-HT may be produced locally by cumulus cells and that it can be actively taken up by mammalian oocytes and embryos, as part of a likely larger serotonergic network possibly regulating various developmental processes much earlier than previously thought.
Follicle stages Antral, Preovulatory
Comment
Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Links
OMIM (Online Mendelian Inheritance in Man: an excellent source of general gene description and genetic information.)
OMIM \ Animal Model
KEGG Pathways
Recent Publications
None
Search for Antibody


created: May 4, 2005, 4:05 p.m. by: hsueh   email:
home page:
last update: May 6, 2005, 9:02 a.m. by: system    email:



Use the back button of your browser to return to the Gene List.

Click here to return to gene search form