Species: mouse
Mutation name: None
type: null mutation
fertility: subfertile
Comment: The meiotic mutant mei-352 is an allele of klp3A and reveals a role for a kinesin-like protein in crossover distribution Page SL, et al . The semi-sterile meiotic mutant mei-352 alters the distribution of meiotic exchanges without greatly affecting their total frequency. We show that the mei-352 mutation is an allele of the klp3A gene, which encodes a kinesin-like protein of the Kinesin-4 family. The semi-sterility observed in mei-352 females results from a known defect of klp3A oocytes in mediating pronuclear fusion. Interestingly, other klp3A alleles also exhibit defects in meiotic recombination similar to those of mei-352. Finally we show that the Klp3A protein localizes within the oocyte nucleus during meiotic prophase, the time at which exchange distribution is established, and extensively colocalizes with DNA. The parallel of the klp3A phenotype with a meiotic defect observed for kar3 mutants in yeast suggests a role for kinesins in early meiosis, and might reflect a previously suggested role for this class of kinesins in chromosome condensation.