NCBI Summary:
Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]
General function
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Cellular localization
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Ovarian function
Oocyte maturation
Comment
CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes. Zhang T et al. (2016) Meiotic resumption (G2/M transition) and progression through meiosis I (MI) are two critical stages for producing fertilization-competent eggs. Here, we report that CenpH, a component of the kinetochore inner plate protein, is responsible for the G2/M transition in meiotic mouse oocytes. Depletion of CenpH using morpholino injection decreased cyclin B1 levels, resulting in an attenuation of MPF activation, and severely compromised the meiotic resumption. CenpH protects cyclin B1 from destruction by competing actions of APC/C(Cdh1) Impaired G2/M transition after CenpH depletion could be rescued by expression of exogenous cyclin B1. Unexpectedly, blocking of CenpH did not affect spindle organization and meiotic cell cycle progression after germinal vesicle breakdown. Our findings reveal a novel role of CenpH in regulating meiotic G2/M transition by acting via the APC/C(Cdh1)-cyclin B1 pathway.//////////////////