NCBI Summary:
ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).[supplied by OMIM, Mar 2008]
General function
DNA Replication
Comment
Cellular localization
Cytoplasmic
Comment
Ovarian function
Cumulus expansion, Luteinization
Comment
Expression and regulation of the differentiation regulators ERBB receptor feedback inhibitor 1 (ERRFI1) and interferon-related developmental regulator 1 (IFRD1) during the periovulatory period in the rat ovary. Li FX et al. (2016) The current study investigated the regulation and the spatiotemporal expression pattern of Errfi1 and Ifrd1, factors that regulate differentiation and cessation of cell division, in the rat ovary during the periovulatory period. Immature female rats (22-23 days old) were injected with pregnant-mare serum gonadotropin to stimulate folliculogenesis, followed by human chorionic gonadotropin (hCG) to induce ovulation. Ovaries, granulosa cells, theca-interstitial cells, or cumulus oocyte complexes (COCs) were collected at various times after hCG administration (n = 3 per time point). Expression analysis revealed that Errfi1 and Ifrd1 were highly induced in the ovary, although their spatiotemporal expression differed: In situ hybridization analysis demonstrated that Errfi1 mRNA expression was initially induced in theca-interstitial cells at 4 and 8 h after hCG, then transitioned to granulosa cells at 12 h, and decreased in newly forming corpora lutea at 24 h. Ifrd1 mRNA, on the other hand, was primarily induced in granulosa cells, and expression remained elevated in newly forming corpora lutea. Interestingly, Errfi1 and Ifrd1 were also expressed in the COC, suggesting a potential role in cumulus cell expansion or oocyte maturation. Inhibition of progesterone or prostaglandin synthesis reduced Errfi1 and Ifrd1 transcription, whereas inhibition of epidermal growth factor signaling inhibited only Errfi1 mRNA abundance. Down-regulation of both genes led to further suppression of progesterone. Our findings thus suggest that the stimulation of Errfi1 and Ifrd1 may be important for theca and granulosa cell differentiation and COC expansion. This article is protected by copyright. All rights reserved.//////////////////
Expression regulated by
LH
Comment
Ovarian localization
Oocyte, Granulosa, Luteal cells
Comment
Follicle stages
Primordial
Comment
Arraztoa JA, et al 2005 reported the identification of genes expressed in primate primordial oocytes.