| lysine (K)-specific demethylase 4D | OKDB#: 5093 |
| Symbols: | KDM4D | Species: | human | ||
| Synonyms: | JMJD2D | Locus: | 11q21 in Homo sapiens |
|
For retrieval of Nucleotide and Amino Acid sequences please go to:
OMIM
Entrez Gene
Mammalian Reproductive Genetics Endometrium Database Resource Orthologous Genes UCSC Genome Browser GEO Profiles new! Amazonia (transcriptome data) new! R-L INTERACTIONS MGI |
| General Comment | |||||
| General function | Enzyme | ||||
| Comment | |||||
| Cellular localization | |||||
| Comment | |||||
| Ovarian function | Early embryo development | ||||
| Comment | Embryonic development following somatic cell nuclear transfer impeded by persisting histone methylation. Matoba S et al. (2014) Mammalian oocytes can reprogram somatic cells into a totipotent state enabling animal cloning through somatic cell nuclear transfer (SCNT). However, the majority of SCNT embryos fail to develop to term due to undefined reprogramming defects. Here, we identify histone H3 lysine 9 trimethylation (H3K9me3) of donor cell genome as a major barrier for efficient reprogramming by SCNT. Comparative transcriptome analysis identified reprogramming resistant regions (RRRs) that are expressed normally at 2-cell mouse embryos generated by in vitro fertilization (IVF) but not SCNT. RRRs are enriched for H3K9me3 in donor somatic cells and its removal by ectopically expressed H3K9me3 demethylase Kdm4d not only reactivates the majority of RRRs, but also greatly improves SCNT efficiency. Furthermore, use of donor somatic nuclei depleted of H3K9 methyltransferases markedly improves SCNT efficiency. Our study thus identifies H3K9me3 as a critical epigenetic barrier in SCNT-mediated reprogramming and provides a promising approach for improving mammalian cloning efficiency.////////////////// | ||||
| Expression regulated by | |||||
| Comment | |||||
| Ovarian localization | Oocyte | ||||
| Comment | |||||
| Follicle stages | |||||
| Comment | |||||
| Phenotypes | |||||
| Mutations | 0 mutations | ||||
| Genomic Region | show genomic region | ||||
| Phenotypes and GWAS | show phenotypes and GWAS | ||||
| Links |
|
| created: | Jan. 21, 2015, 3:55 p.m. | by: |
system email:
home page: |
| last update: | Jan. 21, 2015, 4:04 p.m. | by: | hsueh email: |
Click here to return to gene search form