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General Comment |
NCBI Summary:
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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Mutations |
1 mutations
Species: mouse
Mutation name:
type: naturally occurring
fertility: fertile
Comment: 354 comparison of ovarian contents of akt isoforms among 4 strains of mice. Suzuki O et al. (2014) Strain/individual differences in superovulation efficiency with gonadotropins constitute a serious problem in mouse reproduction (Suzuki et al. 1996 Reprod. Fertil. Dev. 8, 975-980). Because the PI3K/Akt signalling pathway is involved in ovarian folliculogenesis, quantitative difference of protein components in the PI3K/Akt signalling pathway among mouse strains may explain the strain difference in superovulation efficiency. The present study compared ovarian contents of AKT isoforms among 4 strains to examine the involvement of the PI3K/Akt signalling pathway in superovulation. Ovarian protein contents of AKT1, AKT2, and AKT3 isoforms in 4-week-old females of 4 mouse strains were measured by quantitative Western blots with GAPDH as an internal control using whole ovary homogenates (see Suzuki et al. 2011 Exp. Anim. 60, 193-196, for method details). Observed values were compared among strains by 1-way ANOVA after normality (Shapiro-Wilk) and equal variance (Levene) were confirmed. Ovarian protein contents of all 3 AKT isoforms in A/J, C57BL/6N, DBA/2, and C3H/He (arbitrary unit, mean ± s.e.m., n=4) were significantly different among strains by 1-way ANOVA (P<0.05). Ovarian AKT1 contents were 1.01±0.10(a), 0.98±0.07, 0.89±0.03, and 0.84±0.02(b), respectively. Ovarian AKT2 contents were 1.39±0.13(a), 0.74±0.10(b), 1.03±0.07(c), and 0.91±0.19(bc), respectively. Ovarian AKT3 contents were 1.24±0.38, 1.55±0.24(a), 1.11±0.09, and 0.67±0.18(b), respectively. (a-c)Values with different superscripts in each isoform are significantly different by Tukey test (P<0.05). Thus, significant quantitative differences in ovarian AKT isoforms among strains suggest that the ovarian PI3K/Akt signalling pathway acts differently among strains. This activity difference of the pathway may explain the strain difference in superovulation efficiency in mice.//////////////////
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