NCBI Summary:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]
General function
Cytoskeleton organization
Comment
Cellular localization
Cytoskeleton
Comment
Ovarian function
Oocyte maturation
Comment
CENP-T, regulates both G2/M transition and anaphase entry by acting through CDH1 in meiotic oocytes. Wang Y et al. (2020) Oocyte meiotic maturation failure is one of the major causes for female infertility. Meiotic resumption (G2/M transition) and progression through metaphase I (MI) are two critical stages of oocyte meiotic maturation. Here, we report that CENP-T (centromere protein T), an internal kinetochore protein, plays a critical role in meiotic resumption of mouse oocytes. Depletion of CENP-T by siRNA injection increased the CDH1 level, resulting in increased APC/CDH1 activity, and further leading to decreased CCNB1 levels, attenuated MPF activity, and finally severely compromised meiotic resumption. Impaired meiotic resumption caused by CENP-T depletion could be rescued by over-expression of exogenous CCNB1 or knockdown of endogenous CDH1. Over-expression of exogenous CENP-T resulted in decreased CDH1 levels, which accelerated the progression of G2/M transition, and accelerated meiotic cell cycle progression after germinal vesicle breakdown (GVBD). Unexpectedly, spindle organization after GVBD was not affected but the distribution of chromosomes was affected. Our findings reveal a novel role for CENP-T in regulating meiotic progression by acting through CDH1.//////////////////