NCBI Summary:
Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]
General function
Enzyme
Comment
Cellular localization
Cytoplasmic
Comment
Ovarian function
Early embryo development
, Pluripotent cell derivation
Comment
Proteomic analysis of mouse oocytes reveals 28 candidate factors of the 'reprogrammome' Boiani M et al. The oocyte is the only cell of the body that can reprogram transplanted somatic nuclei, and sets the gold standard for all reprogramming methods. Therefore, an in-depth characterization of its proteome holds promise to advance our understanding of reprogramming and germ cell biology. To date, limitations on oocyte numbers and proteomic technology have impeded this task, and the search for reprogramming factors has been conducted in embryonic stem (ES) cells instead. Here, we present the proteome of metaphase II mouse oocytes to a depth of 3699 proteins, which substantially extends the number of proteins identified until now in mouse oocytes and is comparable by size to the proteome of undifferentiated mouse ES cells. Twenty-eight oocyte proteins, also detected in ES cells, match the criteria of our multi-level approach to screen for reprogramming factors, namely: nuclear localization, chromatin modification and catalytic activity. Our oocyte proteome catalog thus advances the definition of the 'reprogrammome', the set of molecules - proteins, RNAs, lipids and small molecules - that enable reprogramming.