Cold stress induces in microorganisms the synthesis of several proteins that are involved in various cellular processes such
as transcription, translation, and recombination. The cold-inducible RNA-binding protein (Cirp) is induced in rodent cells by
mild cold stress (32 degrees C). Cirp consists of an N-terminal RNA-binding domain and a C-terminal gly-rich domain, and
plays an essential role in cold-induced suppression of cell proliferation. Nishiyama et al. (1997) cloned a cDNA encoding
an 18-kD protein with 95.3% amino acid sequence identity to the mouse Cirp protein. CIRBP mRNA was constitutively
expressed in all cell lines examined. In all of the cell lines, the levels of CIRBP mRNA and protein were increased within
12 hours after a temperature downshift from 37 degrees C to 32 degrees C.
Induction of CIRBP expression by cold shock on bovine cumulus-oocyte complexes. Gardela J et al. (2019) The aim of this study was to induce the cold-inducible RNA-binding protein (CIRBP) expression on cumulus-oocyte complexes (COCs) through exposure to a sub-lethal cold shock and determine the effects of hypothermic temperatures during the in vitro maturation of bovine oocytes. Nuclear maturation, cortical granule redistribution and identification of cold-inducible RNA-binding protein (CIRBP) were assessed after 24 hr of in vitro maturation of control (38.5°C) and cold-stressed oocytes (33.5°C). The presence of CIRBP was assessed by Western blot in COCs or denuded oocytes and their respective cumulus cells. Based on the odds ratio, cold-stressed oocytes presented higher abnormal cytoplasmic distribution of cortical granules and nuclear maturation than the control group. Although CIRBP was detected in both control and cold-stressed groups, cold-stressed COCs had 2.17 times more expression of CIRBP than control COCs. However, when denuded oocytes and cumulus cells were assessed separately, CIRBP only was detected in cumulus cells in both groups. In conclusion, cold shock induced CIRBP expression, but it negatively affected nuclear maturation and cortical granule distribution of bovine oocytes. Moreover, the expression of CIRBP was only identified in cumulus cells but not in oocytes.//////////////////
Expression regulated by
LH, Eicosanoids, PGE2
Comment
The midcycle luteinizing hormone (LH) surge stimulates a rise in follicular fluid prostaglandin E2 (PGE2), which is necessary for normal ovulation. To examine PGE2-regulated processes in primate follicles, monkey granulosa cells were cultured with human chorionic gonadotropin (hCG) alone or with hCG and PGE2, and the resulting total RNA was subjected to microarray analysis. Twenty PGE2-regulated mRNAs were identified Seachord CL,et al .