|
Comment |
Ekkehard Brockstedt, et al 2000 reported that comparison of ovary protein patterns, obtained by high resolution
two-dimensional gel electrophoresis from recombinant FSH (rFSH)- and rFSH + human CG (hCG)-treated mice, showed
significant differences in protein spot positions and intensities. Subsequent analysis of one of these proteins was performed
by mass spectrometry, resulting in the identification of the mouse vas deferens protein (MVDP). MVDP, which was absent in
the two-dimensional gel electrophoresis protein pattern of rFSH-primed mice and appeared 3 h after the hCG surge, is a
member of the aldo-keto reductase superfamily and was originally identified in the mouse vas deferens. Northern blot analysis of female mice tissues showed
that mvdp messenger RNA (mRNA) was only present in adrenal glands and in hCG-treated ovaries. In situ hybridization
studies localized the mvdp mRNA unequivocally to ovarian thecal and interstitial cells with an expression profile starting
already 1.5 h, and decreasing 24 h, after LH treatment. In the adrenal glands, mvdp mRNA was not regulated by LH and
localized in the cells of the zona fasciculata. In murine adrenocortical cells, a recent study proposed a detoxifying role of
MVDP. MVDP might fulfill the same function in the ovary; however, because of its strong and early transcriptional induction
by LH, it is also possible that MVDP catalyses another important step during the cascade of events occurring at the time of
ovulation.
|